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 mKate2 Purified Fluorescent Protein- OurProteinFactory

mKate2 Purified Fluorescent Protein

产品编号
PROTN_mKate2N1V00050
¥
1502.9 ¥ 10170.00
规格
50 μg
100 μg
250 μg
1 mg
10 mg
数量
1

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Document
  • NAME

    mKate2

  • DESCRIPTION

    a monomeric far-red fluorescent protein

  • TAG

    His-tag N-Terminus

INTRODUCTION

The excitation and emission maxima of mKate2 are 588 nm and 633 nm, respectively. It’s almost 3-fold brighter than the previously reported mKate and is 10-fold brighter than mPlum. mKate2 exhibits high-brightness, far-red emission spectrum, excellent pH resistance and photostability, low toxicity demonstrated in transgenic Xenopus laevis embryos.

SOURCE

Expressed, purified from Cell-Free System of Protein Factory 1.0

MOLECULAR WEIGHT

29 kDa

FORMULATION

Lyophilized from concentrated solution in PBS, pH 7.4.

PURITY

> 90% SDS-PAGE and FPLC analysis

SPECTROGRAM

ATTRIBUTES

  • Protein
  • EGFP
  • mTagBFP2
  • moxCerulean3
  • zsGreen
  • Clover
  • mVenus
  • SYFP2
  • mKO2
  • tdTomato
  • mScarlet-I
  • mAmetrine
  • PAmCherry2
  • mEos3.2
  • Skylan-S
  • AmCyan1
  • MiCy (Midoriishi-Cyan)
  • ZsYellow1
  • mVenus-Q69M
  • EYFP
  • TurboRFP
  • eqFP611
  • mKate2
  • mNeptune2
  • miRFP670
  • Excitation (nm)
  • 488
  • 399
  • 434
  • 493
  • 505
  • 515
  • 515
  • 551
  • 554
  • 569
  • 406
  • 570
  • 507 / 572
  • 499
  • 453
  • 472
  • 529
  • 515
  • 513
  • 553
  • 559
  • 588
  • 600
  • 642
  • Emission (nm)
  • 507
  • 456
  • 474
  • 505
  • 515
  • 528
  • 527
  • 565
  • 581
  • 593
  • 526
  • 596
  • 516 / 580
  • 511
  • 486
  • 495
  • 539
  • 528
  • 527
  • 574
  • 611
  • 633
  • 650
  • 670
  • Brightness
  • 34
  • 32
  • 36
  • 39
  • 84
  • 53
  • 69
  • 40
  • 95
  • 57
  • 26
  • 13
  • 53 / 18
  • 98
  • 11
  • 25
  • 8
  • 97
  • 45
  • 62
  • 35
  • 25
  • 21
  • 12
  • pKa
  • 6
  • 2.7
  • 3.2
  • 6.1
  • 6
  • 6
  • 5.5
  • 4.7
  • 5.4
  • 6
  • 6.2
  • 5.4 / 5.8
  • 6.6
  • 7
  • 4.4
  • 3.8
  • 5.4
  • 6.3
  • 4.5
  • Maturation
  • 25.0 min
  • 12.0 min
  • 100.4 min
  • 30.0 min
  • 17.6 min
  • 4.1 min
  • 108.0 min
  • 60.0 min
  • 36.0 min
  • 1.8 min
  • 18.0 min
  • 20.0 min
  • 9.6 min
  • 9.0 min
  • 90.0 min
  • 270.0 min
  • 20.0 min
  • 27.0 min
  • 180.0 min
  • Structure
  • Prone to dimerization
  • Prone to dimerization
  • Monomer (A206K)
  • Tetramer
  • Prone to dimerization
  • Monomer (A206K)
  • Monomer (A206K)
  • Monomer
  • Tandem-dimer
  • Monomer
  • Monomer (A206K)
  • Monomer
  • Monomer
  • Tetramer
  • Dimer
  • Tetramer
  • Dimer
  • Tetramer
  • Monomer
  • Prone to dimerization
  • Monomer

PHOTOSTABILITY

  • t1/2(s)
  • 84.0
  • Power
  • Light
  • Mode
  • In Cell
  • Fusion
  • ℃

mKate2 SEQUENCE

mKate2 was derived from mKate with the following mutations: M1_S2insV/V45A/M146T/S158A/K231R

1 MVSELIKENM HMKLYMEGTV NNHHFKCTSE GEGKPYEGTQ TMRIKAVEGG PLPFAFDILA TSFMYGSKTF INHTQGIPDF FKQSFPEGFT WERVTTYEDG
101 GVLTATQDTS LQDGCLIYNV KIRGVNFPSN GPVMQKKTLG WEASTETLYP ADGGLEGRAD MALKLVGGGH LICNLKTTYR SKKPAKNLKM PGVYYVDRRL
201 ERIKEADKET YVEQHEVAVA RYCDLPSKLG HR

USAGE

This material is offered by KANGMA for research, laboratory or further evaluation purposes. RESEARCH USE ONLY & NOT FOR HUMAN US

SPECIFICATIONS

  • Size
  • 50 μg
  • 100 μg
  • 250 μg
  • 1 mg
  • 10 mg
  • Product number
  • PROTN_mKate2N1V00050
  • PROTN_mKate2N2V00050
  • PROTN_mKate2N5V00050
  • PROTN_mKate2N5V00200
  • PROTN_mKate2N5V02000

QUALITY ASSURANCE

The company has strong technical force, advanced production equipment, perfect testing facilities and quality management system, which makes the product appearance consistent and excellent quality.

FOOT
更多联系方式,上海市浦东新区芙蓉花路118弄12-15号楼,请拨打 +1-800-955 -3390or+86-400-770-5500 email to customer@ourproteinfactory.com 周一到周日 0:00-24:00

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