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High Yield (12)-OurProteinFactory

High Yield (12)

产品编号
profac_hyd1210000
价格
11119.20
类别
规格
12 × 10 mL Reaction

DNA定制

数量

1

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蛋白质工厂

Protein Factory 1.0

  • 更简单省时

    Simpler and

    time-saving

  • 更高得率

    High rate

  • 更有活性

    More active

  • 高通量兼容性

    High throughput

    compatibility

世界最高效之蛋白质合成系统

World Most Efficient Protein Synthesis System

Protein

Factory

VER 1.0

蛋白质工厂是一种 复制-转录-翻译 偶联的体外蛋白质合成体系,包含DNA扩增、 mRNA转录和蛋白质翻译必需的全部组分。

Protein Factory is an in vitro protein synthesis system with coupled duplication-transcription-translation reactions, containing all necessary components of DNA duplication, mRNA transcription and protein translation.

全新D2P专利技术

POWERED BY D2P TECHNOLOGY

仅需要加入水和DNA

Only need to add H2O and DNA

用全新的D2P科技,蛋白质工厂能够快速、简便、高通量地生产mg级的,生物活性的蛋白质。

Powered by patented D2P technology, first in history, ProteinFactory can rapidly, simply and in a high-throughput way to generate miligrams of biofunctional proteins.

解决复杂的蛋白质合成难题

Resolve Challenges of Protein Systhesis

产品基于真核细胞,对于合成糖基化/去糖基化、抗体和细胞因子等复杂蛋白更有优势。

The products are based on eukaryotic cells and have advantages in challenging proteins such as glycosylation/deglycosylation, antibodies, cytokines, etc.

更快速

SUPER FAST

2-3 Hours
Room Temperture

蛋白质工厂包含mRNA转录和蛋白质合成必需的所有组分,只需将DNA模板、反应缓冲液和真核细胞提取物混合,置于室温(20 - 30℃)条件下1-2小时,就能得到目标蛋白,不需要复杂操作和其他设备的辅助,为使用者节省大量时间和精力。

Protein Factory encompasses the full elements for mRNA transcription and protein translation. Just add DNA template, buffer to the product powder, at room temperature (20 - 30℃) , 1 to 2 hours, the target protein will be made. No complicated operations or auxiliary equipment required, save your time and energy.

更具放大性

SUPER SCALE

室温条件下,可一步实现从DNA模板到蛋白质的全部过程,不需其他设备,可缩小反应体积至1μL, 更可放大反应规模至 1 吨。

Protein is now made in one step from DNA template, at room temperature, without any equipment, easily enlargeable reaction scales from μL to tons.

更有活性

More active

蛋白质工厂试剂盒基于真核细胞,表达的具有生物学活性的蛋白质种类更多,对于合成糖基化/去糖基化蛋白质、抗体、细胞因子、膜蛋白和大分子蛋白质复合物等复杂蛋白更具优势。

ProteinFactory is based on eukaryotic cells, can express biologically active and well-folded proteins with more advantages, including glycosylated/deglycosylated proteins, antibodies, cytokines, membrane proteins, protein complexes and other complicated proteins.

D2P Tech

更高得率

High yield

蛋白质工厂能够生产毫克-克-公斤级重组蛋白。以萤火虫荧光素酶 (Firefly luciferase, Fluc) 为例,在0.3 μL反应体系中合成的荧 光素酶发出荧光的相对光单位值 (Relative light unit, RLU) 达到了1 × 10e9,对应的蛋白质产量达到了200 μg/mL。

ProteinFactory can produce milligram-gram-kilograms of target recombinant proteins. Using Fluorescent luciferase (Fluc) as example, the relative light unit (RLU) of luciferase synthesized in a 0.3 μL reaction system reaches 1 × 109, with a corresponding protein yield of 200 μg/mL.

更高通量

High Throughput

蛋白质工厂能够对多种目标蛋白 1-10000种 同时进行表达,可快速筛选不同的突变体,可以按照最优的表达条件或者表现最好的目标蛋白进行生产规模的扩大,可以高通量检测目标蛋白的活性,更可用于进行直接的定向优化改造等。

ProteinFactory can express 1-10000 proteins simultaneously, screen mutations, optimize expression conditions or select optimal sequence for large-scale production, can perform high throughput functional assays, and can even be used for direct evolution of your favorite protein.

更特异

More specific

高度特异性的蛋白质表达,整个体系中只有全长目标蛋白被合成,没有被其他蛋白也无任何片段或断裂产生。

No other proteins, no truncation, no fragmentation, only the target full-length protein is made, intact, fresh, and active.

更通用

APPLICATIONS

无论是哪种蛋白质、无论是单体、二聚体、低聚体、异二聚体、复合物,无论何种物种来源,都可以由蛋白质工厂生产。

Proteins, no matter, dimer, oligomer, heteodimer, complex, whatever original source, csn all be made by ProteinFactory.

D2P系统制造的酶活高于细胞表达

D2P-generated Enzymes Activity is Superior to the Cell-Generated Enzyme

基于D2P技术制造的DpnⅠ核酸水解酶的活性高于细胞表达纯化的DpnⅠ蛋白,且D2P技术制造的DpnⅠ不需纯化即可使用,
更加简便。

Enzyme activity of the D2P-based DpnⅠ nuclease is higher than that of the cell-expressing-purified DpnⅠ enzyme.
The D2P-based DpnⅠ enzyme can be used directiy without purification, making protein production even simpler.

专为高得率需求

Specified for High Yield Production

专利暗物质增加得率2-3倍

Dark materials to Increase Yield for 2-3 Times (patented)

高得率促成更好的一步化原位蛋白质合成

High Yield Promoted One-Step In situ Protein Synthesis

ProteinFactory 1.0 High Yield 独有的高得率,不仅提高蛋白质的表达量,更使得目标蛋白的纯化更加容易,使目标蛋白质的纯度更佳。

ProteinFactory 1.0 High Yield provides specialized High Yield technology, promotes expression level of target protein, and further simplify the purification step, making the target protein even purer.

快速3小时反应

Fast 3 Hours Reaction

蛋白质制造只需3小时,方便快速检测活性蛋白质,不 稳定蛋白质等的折叠和相互作用。

Only take 3 hours to make the protein, best for fast analysis of active, unstable proteins, folding, protein-protein interaction.

大范围合成体积 10 µL - 100 mL

Large Range of Reaction Volume 10 µL - 100 mL

独特的匀质化技术,保证得率在极小(10 µL)至极大 (100 mL)的反应体系中均可稳定实现,仅需反应时 充分搅拌混匀。

Patented Homogenization Technology, ensures reproducible High Yield across large range of reaction volumes from the smallest(10 µL) to largest (100 ml), with just through mixing required.

超强的蛋白质合成系统

Stronger System, Better for Large Application

更强的蛋白质合成系统,产生2-3倍更多的蛋白质,满 足您大规模的需求,例如抗原制备、结晶高通量筛选、 动物实验、甚至蛋白质商业生产。

Stronger protein synthesis system with 2-3 times more target protein yield, best fits for your large-scale needs, such as antigen preparation, crystallization, high throughput production, preclinical test, even protein commercial production.

质粒和PCR片段基于的快速目标模板

Compatible Template Preparation for PCR fragments and Plasmid

独有的专利化的快速表达系统,允许通过PCR片段直 接合成蛋白质,可以一次性构建百千种模板。

Patented technology for fast protein expression, allow protein synthesis direct from PCR fragments, with hundreds of templates to be made at once.

大体量的反应体系推荐使用质粒模板,每mL反应体系 仅需0.1 ng 的原始模板。

For large scale reaction, plasmid template is recommended. Only 0.1 ng original plasmid is needed (before amplification) per 1 mL reaction.

高通量目标蛋白表达

High Throughput Target Protein Expression

同样可以一次分别表达96, 384,1536种蛋白, 前所 未有的超高通量。

Unprecedented high throughput capability of expres- sing 96, 384, 1536 proteins at a time.

内标性可定量蛋白质 tag

Quantifiable Internal Tags to Know your Protein Concentration

多种融合蛋白质标签(Tag),eGFP, YFP, Luciferase, 可以直接定量您表达的每一个蛋白质 的浓度,方便您后续的绝对活性校正。

Multiple fusion tags are available that allows direct quantification of target protein concentration, and calibration of subsequent assay results.

Application

大量制备需求的蛋白质;

For proteins needed in large amount;

低表达,难以纯化的蛋白质;

For proteins hard to express, purify in other systems;

商业性的蛋白质生产和制造;

Commercial protein production & sales;

例如抗原制备、结晶、高通量筛选、动物实验;

Use for antigen preparation, crystallization, high-throughput production, preclinical test ect;

标准化的载体

Standardized Vector

  • pD2p pD2p_His8
  • pD2p_V5 pD2p_His8_Flus
  • pD2p_GST pD2p_His8_eGFP
  • pD2p_Flag pD2p_eGFP_Flag
  • pD2p_Fluc pD2p_Histev_eGFP
  • pD2p_MBP pD2p_Flag_eGFP
  • pD2p_eGFP_V5 pD2p_MBPtev_eGFP

世界上第一个DNA-to-Protein (D2P) 技术

World First DNA-to-Protein (D2P) Technology

  • Transcription
  • Translation
  • Protein

超过26项专利赋予的D2P技术,把整个中心法则囊括于内,是世界上唯一的直接从ng级DNA合成mg级蛋白质的技术。

With over 26 patents, the full Central Dogma recapitulated inside, ProteinFactory is world only technology to amplify ng DNA into mg Protein.

定制化服务

Customized Cloning and Assay Service

根据客户对重组蛋白使用目的和要求,可将客户提供的蛋白质的ORF片段,或合成的基因序列,构建至带有不同融合标签的标准化载体中,并对重组蛋白在本试剂盒中的表达水平进行测试和优化(需额外价)。

According to the purpose and requirements of the recombinant protein, services are offered to clone the ORF fragment/gene synthesized of the protein provided by the customer into a standardized vector with different fusion tags, test and optimize the expression level of the recombinant protein in the system (with additional cost).

Protein Factory 表达的蛋白活性高于细胞表达的蛋白质

Protein Factory protein activity is superior to cell-expressed protein

ProteinFactory Expression for Proteins with disulfide bonds

ProteinFactory 制造的酶比活高于细胞表达的商业化酶

ProteinFactory Enzyme Activity is Superior than Commercial Enzyme D2Pfu vs Commerical Pfu DNA Polymerase

包装描述

Kit Description

12 Kit

Protein Factory 1.0 High-Yield (12)

质量的全部保证

Quality Assurance

Protein Factory

  • BP Brand Promise We promise you are 100% satisfied with our products and shopping experience. If not, you may unconditional return any product you purchased from us.
    No No toxic addition All products sold use environmental friendly materials with no toxic additives.
  • S After-sale Warrant We offer the most worry-free after-sales service with full warranty.
    Re 30 Days Return Policy In order to protect our consumers, you are free to return any
    product within 30 days of purchase.

Product Manual Name With Version Data Code

FOOT
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