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 Clover Purified Fluorescent Protein- OurProteinFactory

Clover Purified Fluorescent Protein

Product code
PROTN_CLOVERN1V00050
$
150.29 203.40
Size
50 μg
100 μg
250 μg
1 mg
10 mg
Quantity
1

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Document
  • NAME

    Clover

  • DESCRIPTION

    a dimeric green/yellow

    fluorescent protein
  • TAG

    His-tag N-Terminus

INTRODUCTION

Clover was derived from avGFP with some mutations. The excitation and emission maxima of Clover are 505 nm and 515 nm, respectively. It is reported to be a rapidly-maturing weak dimer with high acid sensitivity.

SOURCE

Expressed, purified from Cell-Free System of Protein Factory 1.0

MOLECULAR WEIGHT

30 kDa

FORMULATION

Lyophilized from concentrated solution in PBS, pH 7.4.

PURITY

> 90% SDS-PAGE and FPLC analysis

SPECTROGRAM

ATTRIBUTES

  • Protein
  • EGFP
  • mTagBFP2
  • moxCerulean3
  • zsGreen
  • Clover
  • mVenus
  • SYFP2
  • mKO2
  • tdTomato
  • mScarlet-I
  • mAmetrine
  • PAmCherry2
  • mEos3.2
  • Skylan-S
  • AmCyan1
  • MiCy (Midoriishi-Cyan)
  • ZsYellow1
  • mVenus-Q69M
  • EYFP
  • TurboRFP
  • eqFP611
  • mKate2
  • mNeptune2
  • miRFP670
  • Excitation (nm)
  • 488
  • 399
  • 434
  • 493
  • 505
  • 515
  • 515
  • 551
  • 554
  • 569
  • 406
  • 570
  • 507 / 572
  • 499
  • 453
  • 472
  • 529
  • 515
  • 513
  • 553
  • 559
  • 588
  • 600
  • 642
  • Emission (nm)
  • 507
  • 456
  • 474
  • 505
  • 515
  • 528
  • 527
  • 565
  • 581
  • 593
  • 526
  • 596
  • 516 / 580
  • 511
  • 486
  • 495
  • 539
  • 528
  • 527
  • 574
  • 611
  • 633
  • 650
  • 670
  • Brightness
  • 34
  • 32
  • 36
  • 39
  • 84
  • 53
  • 69
  • 40
  • 95
  • 57
  • 26
  • 13
  • 53 / 18
  • 98
  • 11
  • 25
  • 8
  • 97
  • 45
  • 62
  • 35
  • 25
  • 21
  • 12
  • pKa
  • 6
  • 2.7
  • 3.2
  • 6.1
  • 6
  • 6
  • 5.5
  • 4.7
  • 5.4
  • 6
  • 6.2
  • 5.4 / 5.8
  • 6.6
  • 7
  • 4.4
  • 3.8
  • 5.4
  • 6.3
  • 4.5
  • Maturation
  • 25.0 min
  • 12.0 min
  • 100.4 min
  • 30.0 min
  • 17.6 min
  • 4.1 min
  • 108.0 min
  • 60.0 min
  • 36.0 min
  • 1.8 min
  • 18.0 min
  • 20.0 min
  • 9.6 min
  • 9.0 min
  • 90.0 min
  • 270.0 min
  • 20.0 min
  • 27.0 min
  • 180.0 min
  • Structure
  • Prone to dimerization
  • Prone to dimerization
  • Monomer (A206K)
  • Tetramer
  • Prone to dimerization
  • Monomer (A206K)
  • Monomer (A206K)
  • Monomer
  • Tandem-dimer
  • Monomer
  • Monomer (A206K)
  • Monomer
  • Monomer
  • Tetramer
  • Dimer
  • Tetramer
  • Dimer
  • Tetramer
  • Monomer
  • Prone to dimerization
  • Monomer

PHOTOSTABILITY

  • t1/2 (s)
  • 50.0
  • Power
  • Light
  • Mode
  • In Cell
  • Fusion

Clover SEQUENCE

Clover was derived from avGFP with the following mutations:

M1_S2insV/S30R/Y39N/S65G/Q69A/F99S/N105T/Y145F/M153T/V163A/I171V/T203H

1 MVSKGEELFT GVVPILVELD GDVNGHKFSV RGEGEGDATN GKLTLKFICT TGKLPVPWPT LVTTFGYGVA CFSRYPDHMK QHDFFKSAMP EGYVQERTIS
101 FKDDGTYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNFNSHN VYITADKQKN GIKANFKIRH NVEDGSVQLA DHYQQNTPIG DGPVLLPDNH
201 YLSHQSALSK DPNEKRDHMV LLEFVTAAGI THGMDELYK

USAGE

This material is offered by KANGMA for research, laboratory or further evaluation purposes. RESEARCH USE ONLY & NOT FOR HUMAN USE.

SPECIFICATIONS

  • Size
  • 50 μg
  • 100 μg
  • 250 μg
  • 1 mg
  • 10 mg
  • Product number
  • PROTN_CLOVERN1V00050
  • PROTN_CLOVERN2V00050
  • PROTN_CLOVERN5V00050
  • PROTN_CLOVERN5V00200
  • PROTN_CLOVERN5V02000

QUALITY ASSURANCE

The company has strong technical force, advanced production equipment, perfect testing facilities and quality management system, which makes the product appearance consistent and excellent quality.

FOOT
More ways to contact,Building #12-15, Furonghua Road, Pudong District, Shanghai, China Zip: 200120, please call +1-800-955-3390or+86-400-770-5500Monday to Sunday 0:00-24:00

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