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 EGFP, Enhanced Green Fluorescent Protein- OurProteinFactory

EGFP, Enhanced Green Fluorescent Protein

产品编号
PROTN_eGFPN1V00050
281.37 1017.00
规格
50 μg
100 μg
250 μg
1 mg
10 mg
数量
1

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Document
  • NAME

    EGFP

  • DESCRIPTION

    Enhanced Green Fluorescent

    Protein Recombinant
  • TAG

    N-terminus His-tag

SYNONYMS

Green fluorescent protein, GFP.

INTRODUCTION

Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish (Aequorea victoria). It shows green fluorescence in short-wave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (EGFP) was derived from GFP with S2insV/F64L/S65T/H231L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 ℃. EGFP permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or a measure of protein-protein interactions.

SOURCE

Expressed, purified from Cell-Free system of Protein Factory 1.0

MOLECULAR WEIGHT

Approximately 30.0 kDa

FORMULATION

Lyophilized from concentrated solution in PBS, pH 7.4.

PURITY

> 95.0% as determined by SDS-PAGE

(a.Coomassie Blue Staining,

b.fluorescence imaging) and

FPLC analysis

SPECTROGRAM

ATTRIBUTES

  • Protein
  • EGFP
  • mTagBFP2
  • moxCerulean3
  • zsGreen
  • Clover
  • mVenus
  • SYFP2
  • mKO2
  • tdTomato
  • mScarlet-I
  • mAmetrine
  • PAmCherry2
  • mEos3.2
  • Skylan-S
  • AmCyan1
  • MiCy (Midoriishi-Cyan)
  • ZsYellow1
  • mVenus-Q69M
  • EYFP
  • TurboRFP
  • eqFP611
  • mKate2
  • mNeptune2
  • miRFP670
  • Excitation (nm)
  • 488
  • 399
  • 434
  • 493
  • 505
  • 515
  • 515
  • 551
  • 554
  • 569
  • 406
  • 570
  • 507 / 572
  • 499
  • 453
  • 472
  • 529
  • 515
  • 513
  • 553
  • 559
  • 588
  • 600
  • 642
  • Emission (nm)
  • 507
  • 456
  • 474
  • 505
  • 515
  • 528
  • 527
  • 565
  • 581
  • 593
  • 526
  • 596
  • 516 / 580
  • 511
  • 486
  • 495
  • 539
  • 528
  • 527
  • 574
  • 611
  • 633
  • 650
  • 670
  • Brightness
  • 34
  • 32
  • 36
  • 39
  • 84
  • 53
  • 69
  • 40
  • 95
  • 57
  • 26
  • 13
  • 53 / 18
  • 98
  • 11
  • 25
  • 8
  • 97
  • 45
  • 62
  • 35
  • 25
  • 21
  • 12
  • pKa
  • 6
  • 2.7
  • 3.2
  • 6.1
  • 6
  • 6
  • 5.5
  • 4.7
  • 5.4
  • 6
  • 6.2
  • 5.4 / 5.8
  • 6.6
  • 7
  • 4.4
  • 3.8
  • 5.4
  • 6.3
  • 4.5
  • Maturation
  • 25.0 min
  • 12.0 min
  • 100.4 min
  • 30.0 min
  • 17.6 min
  • 4.1 min
  • 108.0 min
  • 60.0 min
  • 36.0 min
  • 1.8 min
  • 18.0 min
  • 20.0 min
  • 9.6 min
  • 9.0 min
  • 90.0 min
  • 270.0 min
  • 20.0 min
  • 27.0 min
  • 180.0 min
  • Structure
  • Prone to dimerization
  • Prone to dimerization
  • Monomer (A206K)
  • Tetramer
  • Prone to dimerization
  • Monomer (A206K)
  • Monomer (A206K)
  • Monomer
  • Tandem-dimer
  • Monomer
  • Monomer (A206K)
  • Monomer
  • Monomer
  • Tetramer
  • Dimer
  • Tetramer
  • Dimer
  • Tetramer
  • Monomer
  • Prone to dimerization
  • Monomer

PHOTOSTABILITY

  • t1/2 (s)
  • 174.0
  • 50.1
  • Power
  • 1.5 (mW)
  • Light
  • Arc-lamp
  • Laser
  • Mode
  • Widefield
  • Point Scanning Confocal
  • In Cell
  • Fusion
  • none
  • 37.0

EGFP SEQUENCE

EGFP was derived from avGFP with the following mutations: M1_S2insV/F64L/S65T/H231L

1 MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF
101 FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH
201 YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

USAGE

This material is provided by KANGMA for research, laboratory or further evaluation purposes. RESEARCH USE ONLY & NOT FOR HUMAN USE.

SPECIFICATIONS

  • Size
  • 50 μg
  • 100 μg
  • 250 μg
  • 1 mg
  • 10 mg
  • Product number
  • PROTN_eGFPN1V00050
  • PROTN_eGFPN2V00050
  • PROTN_eGFPN5V00050
  • PROTN_eGFPN5V00200
  • PROTN_eGFPN5V02000

QUALITY ASSURANCE

The company has strong technical force, advanced production equipment, perfect testing facilities and quality management system, which makes the product appearance consistent and excellent quality.

FOOT
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