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 EYFP Purified Fluorescent Protein- OurProteinFactory

EYFP Purified Fluorescent Protein

产品编号
PROTN_EYFPN1V00050
450.87 1017.00
规格
50 μg
100 μg
250 μg
1 mg
10 mg
数量
1

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Document
  • NAME

    EYFP

  • DESCRIPTION

    a weak dimeric yellow

    fluorescent protein
  • TAG

    His-tag N-Terminus

INTRODUCTION

The excitation and emission maxima of EYFP are 513 nm and 527 nm, respectively.

SOURCE

Expressed, purified from Cell-Free System of Protein Factory 1.0

MOLECULAR WEIGHT

30 kDa

FORMULATION

Lyophilized from concentrated solution in PBS, pH 7.4.

PURITY

> 90% SDS-PAGE and FPLC analysis

SPECTROGRAM

ATTRIBUTES

  • Protein
  • EGFP
  • mTagBFP2
  • moxCerulean3
  • zsGreen
  • Clover
  • mVenus
  • SYFP2
  • mKO2
  • tdTomato
  • mScarlet-I
  • mAmetrine
  • PAmCherry2
  • mEos3.2
  • Skylan-S
  • AmCyan1
  • MiCy (Midoriishi-Cyan)
  • ZsYellow1
  • mVenus-Q69M
  • EYFP
  • TurboRFP
  • eqFP611
  • mKate2
  • mNeptune2
  • miRFP670
  • Excitation (nm)
  • 488
  • 399
  • 434
  • 493
  • 505
  • 515
  • 515
  • 551
  • 554
  • 569
  • 406
  • 570
  • 507 / 572
  • 499
  • 453
  • 472
  • 529
  • 515
  • 513
  • 553
  • 559
  • 588
  • 600
  • 642
  • Emission (nm)
  • 507
  • 456
  • 474
  • 505
  • 515
  • 528
  • 527
  • 565
  • 581
  • 593
  • 526
  • 596
  • 516 / 580
  • 511
  • 486
  • 495
  • 539
  • 528
  • 527
  • 574
  • 611
  • 633
  • 650
  • 670
  • Brightness
  • 34
  • 32
  • 36
  • 39
  • 84
  • 53
  • 69
  • 40
  • 95
  • 57
  • 26
  • 13
  • 53 / 18
  • 98
  • 11
  • 25
  • 8
  • 97
  • 45
  • 62
  • 35
  • 25
  • 21
  • 12
  • pKa
  • 6
  • 2.7
  • 3.2
  • 6.1
  • 6
  • 6
  • 5.5
  • 4.7
  • 5.4
  • 6
  • 6.2
  • 5.4 / 5.8
  • 6.6
  • 7
  • 4.4
  • 3.8
  • 5.4
  • 6.3
  • 4.5
  • Maturation
  • 25.0 min
  • 12.0 min
  • 100.4 min
  • 30.0 min
  • 17.6 min
  • 4.1 min
  • 108.0 min
  • 60.0 min
  • 36.0 min
  • 1.8 min
  • 18.0 min
  • 20.0 min
  • 9.6 min
  • 9.0 min
  • 90.0 min
  • 270.0 min
  • 20.0 min
  • 27.0 min
  • 180.0 min
  • Structure
  • Prone to dimerization
  • Prone to dimerization
  • Monomer (A206K)
  • Tetramer
  • Prone to dimerization
  • Monomer (A206K)
  • Monomer (A206K)
  • Monomer
  • Tandem-dimer
  • Monomer
  • Monomer (A206K)
  • Monomer
  • Monomer
  • Tetramer
  • Dimer
  • Tetramer
  • Dimer
  • Tetramer
  • Monomer
  • Prone to dimerization
  • Monomer

PHOTOSTABILITY

  • t1/2 (s)
  • 60.0
  • Power
  • Light
  • Mode
  • In Cell
  • Fusion

EYFP SEQUENCE

EYFP was derived from avGFP with the following mutations:

M1_S2insV/S65G/V68L/S72A/T203Y/H231L

Note: There is inconsistency in the literature regarding the identity of “EYFP”, and whether it contains V68L. Some papers (Lybarger et al. 1998; Patterson et al 2001) equate it with mutant 10C, introduced in Ormö et al 1996, which includes V68L. However, Miyawaki 1997 & 1999 and Nagai 2002 omit V68L, yet another paper from the same group (Rekas 2002) includes V68L (as do most definitions of ‘Venus’ which derives from EYFP). Here we include V68L, and define EYFP as an alias for 10C.

1 MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTFGYGLQ CFARYPDHMK QHDFFKSAMP EGYVQERTIF
101 FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH
201 YLSYQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

USAGE

This material is offered by KANGMA for research, laboratory or further evaluation purposes. RESEARCH USE ONLY & NOT FOR HUMAN USE.

SPECIFICATIONS

  • Size
  • 50 μg
  • 100 μg
  • 250 μg
  • 1 mg
  • 10 mg
  • Product number
  • PROTN_EYFPN1V00050
  • PROTN_EYFPN2V00050
  • PROTN_EYFPN5V00050
  • PROTN_EYFPN5V00200
  • PROTN_EYFPN5V02000

QUALITY ASSURANCE

The company has strong technical force, advanced production equipment, perfect testing facilities and quality management system, which makes the product appearance consistent and excellent quality.

FOOT
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