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DpnⅠ digest DNA when it's meth-
ylated in the recognition site.
DpnⅠ identify GATC sequence and cutting adenine only if it's methylated.
in E. coli, due to the methylation modification
restriction system,dam methylation enzyme methylate
DNA.
So that the plasmid extracted from E. coli dam+ strain
contains methlated GATC and thus can be
recognized by DpnI and cut off.
D2P-DpnⅠ is the world's first commercially available produced by D2P system.
D2P-DpnⅠ can be used directly form D2P system without purification.
One unit is defined as the amount of enzyme required to digest 1 μg pUC19 DNA (dam
methylated)
in 1 hour at 37℃ in a total reaction volume of 50 μl.
Reaction Condition
D2P-DpnⅠ can be directly applied into PCR product without
adding extra buffer.incubate at 37℃.
D2P-DpnⅠ is produced form our D2P system with DpnⅠ gene from Streptococcus pneumoniae.
Storage Temperature:-20℃
Storage buffer:50% glycerol
Heat inactivation:80℃ for 20 min
Site directed mutagenesis. Restriction enzyme.
With methylated plasmid template and non-methylated DNA product in PCR, DpnI will specifically cleave plasmid template. So that the influence of the original template can be eliminated, only remaining target DNA product.
D2P-DpnⅠ is a mixture of IVTT product.
Method: Transform D2P-DpnⅠ directly into
Result: no colony grows in the plates.
Conclusion: the DNA remaining in D2P-DpnⅠ doesn't form any colony.
D2P-DpnⅠ 37℃ 1h
D2P-DpnⅠ 37℃ 2h
Positive control plate for transformed pUC19
Stronger Activity
Digest 1ug pUC19 plasmid in 37ᵒC 1h with D2P-DpnⅠ
10 U D2P-DpnⅠ
3 U D2P-DpnⅠ
negative control
DpnⅠ限制性内切酶
DpnⅠ Restriction Enzyme
细节展示
Improves sample safety with
non-cytotoxic materials
With the external capping technology,
the advantages of aseptic technique are
more prominent.
Standpipe, convenient using on
the workbench
产品规格
98%
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