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 DpnⅠ Restriction Enzyme- OurProteinFactory

DpnⅠ Restriction Enzyme

Product code
PROTN_DpnIN10V00020
$
141.25
Size
10 x 20 μL
Quantity
1

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DpnⅠ Restriction Enzyme

DpnⅠ限制性内切酶

DpnⅠ digest DNA when it's meth-
ylated in the recognition site.

Details

详情

DpnⅠ identify GATC sequence and cutting adenine only if it's methylated.

in E. coli, due to the methylation modification restriction system,dam methylation enzyme methylate
DNA.

So that the plasmid extracted from E. coli dam+ strain contains methlated GATC and thus can be
recognized by DpnI and cut off.

World First Cell Free Enzyme

世界首个无细胞酶

D2P-DpnⅠ is the world's first commercially available produced by D2P system.

D2P-DpnⅠ can be used directly form D2P system without purification.

Cell
Free

Product Description

产品介绍

One unit is defined as the amount of enzyme required to digest 1 μg pUC19 DNA (dam methylated)
in 1 hour at 37℃ in a total reaction volume of 50 μl.

Reaction Condition
D2P-DpnⅠ can be directly applied into PCR product without adding extra buffer.incubate at 37℃.

D2P-DpnⅠ is produced form our D2P system with DpnⅠ gene from Streptococcus pneumoniae.

贮存条件

Storage Temperature:-20℃

Storage buffer:50% glycerol

Heat inactivation:80℃ for 20 min

产品应用

Site directed mutagenesis. Restriction enzyme.

With methylated plasmid template and non-methylated DNA product in PCR, DpnI will specifically cleave plasmid template. So that the influence of the original template can be eliminated, only remaining target DNA product.

Clean

D2P-DpnⅠ is a mixture of IVTT product.
Method: Transform D2P-DpnⅠ directly into

Result: no colony grows in the plates.
Conclusion: the DNA remaining in D2P-DpnⅠ doesn't form any colony.

6 μL 2.5 μL 1.25 μL

D2P-DpnⅠ 37℃ 1h

D2P-DpnⅠ 37℃ 2h

Positive control plate for transformed pUC19

Stronger Activity

Digest 1ug pUC19 plasmid in 37ᵒC 1h with D2P-DpnⅠ

10 U D2P-DpnⅠ

3 U D2P-DpnⅠ

negative control

DpnⅠ Restriction Enzyme

DpnⅠ限制性内切酶

用于单点突变分子克隆、重组法分子克隆或其他实验的理想产品,快速简单的即用型内切酶,每个反应成本更低
Higher efficiency: quick achievement, quality assurance

DpnⅠ Restriction Enzyme

DpnⅠ限制性内切酶

Package Details

细节展示

采用无细胞毒性材料,提升样品安全性

Improves sample safety with
non-cytotoxic materials

采用外旋盖技术,无菌技术优势更为突出

With the external capping technology,
the advantages of aseptic technique are
more prominent.

立管,方便工作台使用

Standpipe, convenient using on
the workbench

Product Specifications

产品规格

FOOT
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